ElemBio AVITI24 Sequencing Compatibility

ElemBio AVITI24 Sequencing Compatibility

ElemBio AVITI24 Sequencing Compatibility

For Cloudbreak Freestyle chemistry, Element oligonucleotides include sequencing primers that are compatible with standard Nextera, TruSeq, and small RNA libraries.
Libraries with sequencing primer binding sites that do not meet these requirements must use custom primers.

Assays Requiring Additional Processing

Incompatible assay or workflows

Vendor	Workflow	Kit example	Expected result	Note
IDT	PCR-free	xGen™ UDI-UMI Adapters, 16 rxn, 96rxn	No polonies (PCR-free workflow only)	PCR-plus workflow is compatible
IDT	Enzyme based normalization	xGen™ Normalase™ Module 96rxn	No polonies
Illumina	Bead-based normalization	Nextera XT DNA Library Preparation Kit (24, 96 samples)	No polonies	The final library is compatible if it does NOT go through bead-based normalization
		TruSight Tumor 170 NextSeq Kit (24 Sample Library Prep Kit)
		TruSight Oncology 500 DNA Kit (48 Sample Library Prep Kit Only)
		TruSight Oncology 500 DNA/RNA Bundle (24 Sample Library Prep Kit Only)

Assays that may show variable performance

Vendor	Workflow	Kit example	Expected result
Illumina	Library prep workflow with amplification module using “PPC” reagent	Illumina DNA Prep with Enrichment	Low or variable density
		Illumina RNA Prep with Enrichment (L) Tagmentation
		TruSeq ChIP Library Prep
		TruSeq DNA Nano
		TruSeq RNA Exome
		TruSeq RNA Library Prep Kit v2
		TruSeq Stranded Total RNA
		TruSeq Stranded Total RNA with Ribo-Zero rRNA Depletion
		TruSeq Stranded mRNA
		TruSight RNA Fusion Panel
		TruSight RNA Pan-Cancer

Custom Sequencing Primers

Assays requiring custom sequencing primers

Vendor	Assay Kit	Custom Primer Requirements
10x Genomics	Single Cell Gene Expression Flex	Spike-in corresponding small RNA sequencing primers for Index1 and Read2 primers; Sequencing cartridge Lot 2409210001 and higher include updated primers
10x Genomics	Visium Spatial Gene Expression for FFPE
10x Genomics	Visium HD
10x Genomics	Libraries with Small RNA adapter for Read 2 – “TS” index set
Revvity	NEXTFLEX Small RNA-Seq Kit v4 with UDIs
Illumina	TruSeq Small RNA Library Prep	Spike-in corresponding small RNA sequencing primers for Index1, Read1, and Read2 primers; Sequencing cartridge Lot 2409210001 and higher include updated primers
Illumina	Illumina PCR-free Library Prep	Custom sequencing primer for Read1
NEB	NEBNext Small RNA library	Custom sequencing primer for Read1
Custom	n/a	Custom sequencing primers accordingly

Cloudbreak Freestyle kits updated, by part number

Product Number	Product Name	Kit updated to include small RNA sequencing primers Read1, Read2, and Index1
860-00014	AVITI 2×75 Sequencing Kit Cloudbreak FS Medium Output	Sequencing cartridge Lot 2409210001 and higher include updated primers
860-00015	AVITI 2×75 Sequencing Kit Cloudbreak FS High Output
860-00011	AVITI 2×150 Sequencing Kit Cloudbreak FS Low Output
860-00012	AVITI 2×150 Sequencing Kit Cloudbreak FS Medium Output
860-00013	AVITI 2×150 Sequencing Kit Cloudbreak FS High Output
860-00016	AVITI 2×300 Sequencing Kit Cloudbreak FS Medium Output
860-00017	AVITI 2×300 Sequencing Kit Cloudbreak FS High Output