ElemBio AVITI24 Sequencing Compatibility
ElemBio AVITI24 Sequencing Compatibility
- For Cloudbreak Freestyle chemistry, Element oligonucleotides include sequencing primers that are compatible with standard Nextera, TruSeq, and small RNA libraries.
- Libraries with sequencing primer binding sites that do not meet these requirements must use custom primers.
Assays Requiring Additional Processing
Incompatible assay or workflows
Vendor Workflow Kit example Expected result Note
IDT PCR-free xGen™ UDI-UMI Adapters, 16 rxn, 96rxn No polonies (PCR-free workflow only) PCR-plus workflow is compatible
IDT Enzyme based normalization xGen™ Normalase™ Module 96rxn No polonies
Illumina
Bead-based normalizationNextera XT DNA Library Preparation Kit (24, 96 samples)
No polonies
The final library is compatible if it does NOT go through bead-based normalization
TruSight Tumor 170 NextSeq Kit (24 Sample Library Prep Kit)
TruSight Oncology 500 DNA Kit (48 Sample Library Prep Kit Only)
TruSight Oncology 500 DNA/RNA Bundle (24 Sample Library Prep Kit Only)
Assays that may show variable performance
Vendor Workflow Kit example Expected result
Illumina
Library prep workflow with amplification module using “PPC” reagentIllumina DNA Prep with Enrichment
Low or variable density
Illumina RNA Prep with Enrichment (L) Tagmentation
TruSeq ChIP Library Prep
TruSeq DNA Nano
TruSeq RNA Exome
TruSeq RNA Library Prep Kit v2
TruSeq Stranded Total RNA
TruSeq Stranded Total RNA with Ribo-Zero rRNA Depletion
TruSeq Stranded mRNA
TruSight RNA Fusion Panel
TruSight RNA Pan-Cancer
Custom Sequencing Primers
Assays requiring custom sequencing primers
Vendor Assay Kit Custom Primer Requirements
10x Genomics Single Cell Gene Expression Flex
Spike-in corresponding small RNA sequencing primers for Index1 and Read2 primers;
Sequencing cartridge Lot 2409210001 and higher include updated primers
10x Genomics Visium Spatial Gene Expression for FFPE
10x Genomics Visium HD
10x Genomics Libraries with Small RNA adapter for Read 2 – “TS” index set
Revvity NEXTFLEX Small RNA-Seq Kit v4 with UDIs
Illumina TruSeq Small RNA Library Prep Spike-in corresponding small RNA sequencing primers for Index1, Read1, and Read2 primers;
Sequencing cartridge Lot 2409210001 and higher include updated primers
Illumina Illumina PCR-free Library Prep Custom sequencing primer for Read1
NEB NEBNext Small RNA library Custom sequencing primer for Read1
Custom n/a Custom sequencing primers accordingly
Cloudbreak Freestyle kits updated, by part number
| Product Number | Product Name | Kit updated to include small RNA sequencing primers Read1, Read2, and Index1 |
|---|---|---|
| 860-00014 | AVITI 2×75 Sequencing Kit Cloudbreak FS Medium Output | Sequencing cartridge Lot 2409210001 and higher include updated primers |
| 860-00015 | AVITI 2×75 Sequencing Kit Cloudbreak FS High Output | |
| 860-00011 | AVITI 2×150 Sequencing Kit Cloudbreak FS Low Output | |
| 860-00012 | AVITI 2×150 Sequencing Kit Cloudbreak FS Medium Output | |
| 860-00013 | AVITI 2×150 Sequencing Kit Cloudbreak FS High Output | |
| 860-00016 | AVITI 2×300 Sequencing Kit Cloudbreak FS Medium Output | |
| 860-00017 | AVITI 2×300 Sequencing Kit Cloudbreak FS High Output |
