FAQs

Administration

  1. How do I get started working with the NGS services? (User Manual)
  2. How do I fill out the forms on NGS Core LIMS?
  3. How do I download my sequencing data?
  4. How do I pay my fees? (Billing Procedures)
  5. 《樣本製備風險告知同意書_standard》   《樣本製備風險告知同意書_turbo DNase ver.》
  6. (S-L) Index Sheet for Ready-to-Seq Library 
  7. Performance parameters for Illumina Sequencing System

Experiment Related

  1. How to select index for illumina sequencing?
  2. Index hopping issue on patterned flowcells and methods of mitigation. (NextSeq2000 / iSeq)
  3. Commonly used Illumina library prep kits and protocols.

  4. RNA-seq data processing: HTSream and FASTP
    Why does FASTQC show unexpectedly high sequence duplication levels (PCR-duplicates)?
    (From UC Davis Genome Center)

  5. Adaptor trimming for TruSeq DNA Methylation
  6. Illumina PhiX control is GI:9626372 RefSeq:NC_001422.
  7. Amplicon-seq design for Illumina library architecture
  8. Illumina Chemistry and imaging