{"id":3006,"date":"2022-03-23T18:34:48","date_gmt":"2022-03-23T10:34:48","guid":{"rendered":"http:\/\/ngs.biodiv.tw\/NGSCore\/?p=3006"},"modified":"2026-03-26T17:28:51","modified_gmt":"2026-03-26T09:28:51","slug":"adaptor-trimming-for-truseq-dna-methylation","status":"publish","type":"post","link":"https:\/\/ngs.biodiv.tw\/NGSCore\/2022\/03\/23\/adaptor-trimming-for-truseq-dna-methylation\/","title":{"rendered":"Adaptor trimming for TruSeq DNA Methylation"},"content":{"rendered":"<h4>1.<\/h4>\n<h4>TruSeq DNA Methylation\u6240\u4f7f\u7528\u7684adapter\u5e8f\u5217\u8207 TruSeq Single Index\u7cfb\u7d71\u76f8\u540c\uff0c bcl2fastq v2.2 guide\u5728\u62c6\u5206\u904e\u7a0b\u4e2d\u8a72\u8edf\u9ad4\u5224\u65b7adapter\u5e8f\u5217\u7684\u56b4\u8b39\u5ea6(adapter stringency)\u5b9a\u7fa9\u5982\u4e0b\uff1a<\/h4>\n<blockquote>\n<h4>The minimum match rate that triggers masking or trimming. This value is calculated as MatchCount \/ (MatchCount + MismatchCount). &#8230; The default value of\u00a0<span style=\"color: #ff0000;\"><strong>0.9<\/strong><\/span>\u00a0indicates that only reads with\u00a0<span style=\"color: #ff0000;\"><strong>&gt;<\/strong> <strong>90%<\/strong><\/span>\u00a0sequence identity with the adapter are trimmed.<\/h4>\n<h4>(page. 14,\u00a0<a href=\"https:\/\/support.illumina.com\/content\/dam\/illumina-support\/documents\/documentation\/software_documentation\/bcl2fastq\/bcl2fastq2-v2-20-software-guide-15051736-03.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">bcl2fastq2-v2-20-software-guide<\/a>)<\/h4>\n<\/blockquote>\n<h4>\u7531\u6b64\u7814\u5224\uff0c<br \/>\n25 mer \u9700\u6709 23 mer \u7b26\u5408\u5206\u6790\u8005\u7d66\u4e88\u7684\u5e8f\u5217 (92%)<br \/>\n33 mer \u9700\u6709 30 mer \u7b26\u5408\u5206\u6790\u8005\u7d66\u4e88\u7684\u5e8f\u5217 (90.9%)<br \/>\n\u624d\u6703\u88abtrim\uff0c\u5169\u8005\u6bd4\u4f8b\u50c5\u4e9b\u5fae\u5dee\u7570\u3002<\/h4>\n<h4>2.<\/h4>\n<h4>\u8d77\u521d\u7531\u65bcTruSeq DNA Methylation\u7684\u5efa\u8b70\u8b80\u9577\u8f03\u77ed( 2\u00d775 or 2\u00d7100bp)\uff0c\u4f5c\u70ba\u56e0\u61c9\uff0c\u63d0\u4f9b\u7684trimming\u5e8f\u5217\u4ea6\u8f03\u77ed\uff1b\u82e5\u4f7f\u7528\u8f03\u9577\u7684\u8b80\u9577\u59822\u00d7150\u6216\u4ee5\u4e0a\uff0c\u4ea6\u53ef\u4ee5\u4f7f\u752833 mer\uff1b\u4f46\u6574\u9ad4\u800c\u8a00\uff0c25 mer\u5c31\u53ef\u4ee5\u9054\u5230trim adapter\u7684\u6548\u679c\u3002<br \/>\nRecommended read length: <a href=\"https:\/\/emea.illumina.com\/library-prep-array-kit-selector\/kits-and-arrays\/truseq-dna-methylation.html\" target=\"_blank\" rel=\"noreferrer noopener\">TruSeq DNA Methylation (illumina.com)<\/a><\/h4>\n","protected":false},"excerpt":{"rendered":"<p>1. TruSeq DNA Methylation\u6240\u4f7f\u7528\u7684adapter\u5e8f\u5217\u8207 TruSeq Single I [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[22],"tags":[],"class_list":["post-3006","post","type-post","status-publish","format-standard","hentry","category-faqs"],"_links":{"self":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/posts\/3006","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/comments?post=3006"}],"version-history":[{"count":15,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/posts\/3006\/revisions"}],"predecessor-version":[{"id":4584,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/posts\/3006\/revisions\/4584"}],"wp:attachment":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/media?parent=3006"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/categories?post=3006"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/tags?post=3006"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}