{"id":2260,"date":"2000-03-14T01:46:39","date_gmt":"2000-03-13T17:46:39","guid":{"rendered":"http:\/\/ngs.biodiv.tw\/NGSCore\/?p=2260"},"modified":"2026-03-26T17:28:52","modified_gmt":"2026-03-26T09:28:52","slug":"pacbio-sequel","status":"publish","type":"post","link":"https:\/\/ngs.biodiv.tw\/NGSCore\/2000\/03\/14\/pacbio-sequel\/","title":{"rendered":"PacBio \u2013 Sequel"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><img fetchpriority=\"high\" decoding=\"async\" width=\"870\" height=\"1024\" src=\"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-content\/uploads\/2021\/03\/PacBio_Table-870x1024.png\" alt=\"\" class=\"wp-image-2261\" srcset=\"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-content\/uploads\/2021\/03\/PacBio_Table-870x1024.png 870w, https:\/\/ngs.biodiv.tw\/NGSCore\/wp-content\/uploads\/2021\/03\/PacBio_Table-255x300.png 255w, https:\/\/ngs.biodiv.tw\/NGSCore\/wp-content\/uploads\/2021\/03\/PacBio_Table-768x904.png 768w, https:\/\/ngs.biodiv.tw\/NGSCore\/wp-content\/uploads\/2021\/03\/PacBio_Table-1306x1536.png 1306w, https:\/\/ngs.biodiv.tw\/NGSCore\/wp-content\/uploads\/2021\/03\/PacBio_Table.png 1700w\" sizes=\"(max-width: 870px) 100vw, 870px\" \/><\/figure>\n\n\n\n<p>With SMRT (Single Molecule Real-Time) technology, PacBio Sequel enables the real-time detection of DNA synthesis, which is done by incorporation of phospholinked nucleotides, and allows DNA polymerase to complete natural process of elongation. The events of DNA synthesis occurs in the bottom of ZMWs (zero-mode waveguides), allowing the illumination to be identified from background noises such as unincorporated free nucleotides.<\/p>\n<p>PacBio Sequel system provides long-read sequencing, which facilitates assemblies of difficult genomic regions. It also offers benefits such as uniform coverage and high consensus accuracy, and becomes a powerful technology for high quality de novo genome study, full-length transcriptome investigation, haplotype phasing, and long length amplicon research.<\/p>\n\n\n\n<figure class=\"wp-block-embed-youtube wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe title=\"Introduction to PacBio Highly Accurate Long-Read Sequencing\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/lT3NqhKD840?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<figure class=\"wp-block-embed-youtube wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe title=\"PacBio Sequencing \u2013 How it Works\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/_lD8JyAbwEo?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20],"tags":[],"class_list":["post-2260","post","type-post","status-publish","format-standard","hentry","category-sequencing"],"_links":{"self":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/posts\/2260","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/comments?post=2260"}],"version-history":[{"count":2,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/posts\/2260\/revisions"}],"predecessor-version":[{"id":4651,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/posts\/2260\/revisions\/4651"}],"wp:attachment":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/media?parent=2260"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/categories?post=2260"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/tags?post=2260"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}