{"id":2239,"date":"2021-03-14T00:49:22","date_gmt":"2021-03-13T16:49:22","guid":{"rendered":"http:\/\/ngs.biodiv.tw\/NGSCore\/?page_id=2239"},"modified":"2021-03-14T01:35:00","modified_gmt":"2021-03-13T17:35:00","slug":"sequencer","status":"publish","type":"page","link":"https:\/\/ngs.biodiv.tw\/NGSCore\/sequencer\/","title":{"rendered":"Sequencer"},"content":{"rendered":"\n\n\n\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
<\/td>Element Bio \u2013 AVITI24 System<\/a><\/strong>
\nThe AVITI24 System with Teton\u2122 CytoProfiling simultaneously measures a diverse array of molecular features in up to 1 million cells per flow cell for deep multiomic profiling of adherent cells or cell suspensions. From a single sample, you can analyze RNA, proteins, and cell morphology in 24 hours.
\n
\nOn the same system, the AVITI24 delivers high-quality next\u2011generation sequencing (NGS) data with uncompromising performance, flexibility, and affordability, and access to a range of assays from whole-genome sequencing to targeted panels. <\/td>\n<\/tr>\n
<\/td>Illumina \u2013 NextSeq 2000<\/a><\/strong>
\nThe latest NGS system from Illumina offers innovative design features, advanced chemistry, simplified bioinformatics, and an intuitive workflow that enable the widest range of applications on a benchtop sequencing system.<\/td>\n<\/tr>\n
<\/td>Oxford Nanopore Technology \u2013 GridION<\/a><\/strong>
\nGridION is a desktop device that allows simple scaling of MinION and Flongle Flow Cells. Users are able to run up to five flow cells simultaneously, enabling the generation of as much as 150Gb (assuming 30Gb per flow cell) of sequence data if using all five MinION flow cells at the same time.
\nIt offers the same real-time, on-demand, long-read, direct DNA or RNA sequencing as other nanopore platforms. Added to this, the integrated compute for onboard, real-time analysis provides capacity well in excess of that required for basecalling alone.<\/td>\n<\/tr>\n
<\/td>Oxford Nanopore Technology \u2013 ONT P2 solo<\/a><\/strong>
\nPromethION 2 devices for flexible, high-yield sequencing, in a compact, accessible design. Utilising flow cells that generate hundreds of gigabases, enable PromethION-scale benefits in small to medium sized labs. Suitable for users with lower sample processing requirements, up to ~200 flow cells per year.<\/td>\n<\/tr>\n
\"\"<\/td>PacBio \u2013 Sequel & Sequel IIe<\/a><\/strong>
\nThe Sequel System provides high throughput sequencing base on SMRT technology.The core of the Sequel System is the capacity of its redesigned SMRT Cells, which contain one million zero-mode waveguides (ZMWs) at launch, compared to 150,000 ZMWs in the PacBio RS II. Active individual polymerases are immobilized within the ZMWs, providing windows to observe and record DNA sequencing in real time.<\/td>\n<\/tr>\n
\"\"<\/td><\/a><\/strong>
\nIllumina \u2013 HiSeq 2500 (Retired)<\/strong>
\nThe HiSeq 2500 System is a powerful sequencing system with the flexibility to perform multiple applications. High-quality data using proven Illumina SBS chemistry has made it the instrument of choice for major genome centers and research institutions throughout the world.<\/td>\n<\/tr>\n
<\/td>Illumina \u2013 MiSeq<\/a><\/strong>
\nThe MiSeq System facilitates your research with a wide range of sequencing applications. It is capable of automated paired-end reads and up to 15 Gb per run, delivering over 600 bases of sequence data per read. The library prep kits that it uses are optimized for a variety of applications, including targeted gene, small genome, and amplicon sequencing, 16S metagenomics, and more.<\/td>\n<\/tr>\n
\"\"<\/td>Roche 454 GS FLX PLUS (Retired)<\/strong>
\nIt is upgraded to FLX+, the longest read length capacity up to 1kb, generating over 700 Mb to around 1Gb for shotgun genome sequencing. This is very useful for de novo assembly (genome and transcriptome) and long amplicon sequencing studies. There are options of shotgun sequencing for gDNA and RNA, as well as Long Paired-end sequencing designated at 3, 8, and 20 kb.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2239","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/pages\/2239","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/comments?post=2239"}],"version-history":[{"count":3,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/pages\/2239\/revisions"}],"predecessor-version":[{"id":4142,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/pages\/2239\/revisions\/4142"}],"wp:attachment":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/media?parent=2239"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}