{"id":148,"date":"2016-09-26T12:24:08","date_gmt":"2016-09-26T04:24:08","guid":{"rendered":"http:\/\/140.109.28.108\/brcasngscore\/?page_id=148"},"modified":"2025-08-05T15:56:45","modified_gmt":"2025-08-05T07:56:45","slug":"service-flow","status":"publish","type":"page","link":"https:\/\/ngs.biodiv.tw\/NGSCore\/service-flow\/","title":{"rendered":"Service Flow"},"content":{"rendered":"\t\t
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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t<\/i>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\tSample Requirement<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Service Flow<\/span><\/strong><\/p>\n\n

\"NGS_ServiceWorkflow\"<\/a>
\n\n\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
1<\/td>Inquire
\nNGS Service<\/td>		Contact Core Manager to inquire service application of interest.<\/td>\n<\/tr>\n
2<\/td>Project
\nConsultation<\/td>		Project discussion regarding sample organism, sequence type, and scale of data required.
\nPlease also provide sample information for visual quality inspection.<\/td>\n<\/tr>\n
3<\/td>On-line
\nApplication<\/td>		Log-in LIMS and complete on-line application.
\n<\/td>\n<\/tr>\n
4<\/td>Submit Sample
\nand Forms<\/td>		Download Sequencing Application Form and Sample Submission Form, and have the form signed by PI for project approval.
\nSend approved samples and forms to core, signed in by Core Manager for project establishment.<\/td>\n<\/tr>\n
5<\/td>Sample QC<\/td>Check DNA\/RNA concentration, purity, and quality.<\/td>\n<\/tr>\n
6<\/td>Library
\nConstruction<\/td>		Application-specific procedure; PCR amplification.<\/td>\n<\/tr>\n
7<\/td>Library QC<\/td>Check library size range and concentration; qPCR normalization.<\/td>\n<\/tr>\n
8<\/td>Sequencing<\/td>Application dependent sequencing (SR vs PE; read length of choice)<\/td>\n<\/tr>\n
9<\/td>Data
\nPreparation<\/td>		Demultiplexing; read folder generation; summary file. (Optional: assembly or mapping analysis upon request.)<\/td>\n<\/tr>\n
10<\/td>Return Data
\nto User<\/td>		Transfer data\/files by retrieval server FTP. Portable HD copy is available only if necessary.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/p>\n
\n

\u5728\u63d0\u51fa\u7dda\u4e0a\u7533\u8acb\u4e4b\u524d\uff0c\u8acb\u5148\u806f\u7d61 NGS Core Manager<\/a>\uff0c\u4e26\u63d0\u4f9b\u6a23\u54c1\u660e\u7d30\uff0c\u5305\u62ec\u6fc3\u5ea6\u3001\u7d14\u5ea6\u3001\u81a0\u5716\u3001\u7b49\u76f8\u95dc\u8cc7\u6599\uff0c\u4ee5\u4fbf\u91dd\u5c0d\u4f60\u7684\u5be6\u9a57\u9700\u6c42\u9032\u884c\u8a0e\u8ad6\u8207\u898f\u5283\u3002\u5f85\u6240\u6709\u9700\u6c42\u90fd\u7d93\u78ba\u8a8d\u904e\u5f8c\uff0c\u624d\u53ef\u4ee5\u767b\u5165 LIMS \u586b\u5beb\u7dda\u4e0a\u7533\u8acb\u8868\u3002<\/span><\/p>\n

\u4f60\u7684\u7dda\u4e0a\u7533\u8acb\u8868\u901a\u904e\u5be9\u6838\u5f8c\uff0c\u5373\u53ef\u9810\u7d04\u9001\u4ef6\u3002\u8acb\u5148\u4e0b\u8f09\u5b9a\u5e8f\u670d\u52d9\u7533\u8acb\u8868\u8207\u6a23\u54c1\u660e\u7d30\u8868\uff0c\u5c07\u8868\u55ae\u5217\u5370\u51fa\u4f86\u7d66 PI \u7c3d\u540d\u5f8c\uff0c\u624d\u80fd\u7b97\u662f\u6709\u6548\u6587\u4ef6\u3002\u5e36\u8457\u4f60\u7684\u6a23\u54c1\u8207\u7533\u8acb\u6587\u4ef6\uff08\u5305\u542b\u5b9a\u5e8f\u7533\u8acb\u8868\u3001\u6a23\u54c1\u660e\u7d30\u8868\u3001\u6a23\u54c1\u88dc\u5145\u8cc7\u6599<\/a>\uff09\u5373\u53ef\u4f9d\u7d04\u597d\u7684\u6642\u9593\u524d\u5f80\u9001\u4ef6\u3002<\/span><\/p>\n

\u6a23\u54c1\u9001\u9054\u4e14\u6848\u4ef6\u6210\u7acb\u4e4b\u5f8c\uff0c\u4f60\u53ef\u4ee5\u96a8\u6642\u767b\u5165 LIMS \u67e5\u8a62\u6240\u6709\u6848\u4ef6\u7684\u57f7\u884c\u9032\u5ea6\uff0c\u4e5f\u53ef\u4ee5\u5728\u7dda\u4e0a\u67e5\u770b\u7d50\u679c\u6216\u4e0b\u8f09\u5831\u544a\u3002<\/span><\/p>\n

\u6a23\u54c1\u88fd\u5099\u597d\u5f8c\uff0c\u6211\u5011\u6703\u4f9d\u5e8f\u5b89\u6392\u4e0a\u6a5f\uff1b\u4e26\u5728\u5b8c\u6210\u5b9a\u5e8f\u8207\u5206\u6790\u5f8c\uff0c\u901a\u77e5\u4f60\u53d6\u56de\u5b9a\u5e8f\u7d50\u679c\u3002<\/span><\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t
\n\t\t\t\t\t\t\t\t\tNGS_Core_LIMS_UserManual_181030<\/a>\n

Download<\/a><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

Sample Requirement Sample Preparation Sample Submission […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-148","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/pages\/148","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/comments?post=148"}],"version-history":[{"count":59,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/pages\/148\/revisions"}],"predecessor-version":[{"id":6049,"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/pages\/148\/revisions\/6049"}],"wp:attachment":[{"href":"https:\/\/ngs.biodiv.tw\/NGSCore\/wp-json\/wp\/v2\/media?parent=148"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}