Instruments

Illumina – HiSeq 2500 and MiSeq

Our current models include two HiSeq 2500 and one MiSeq sequencers. Sequencing can be single-end (SR) or paired-end (PE) format. Read length can be defined according to the length most suitable to the desired application. Mate-pair library is standard between 2-10kb, optional to 15 kb of jump distance.

 

PacBio – Sequel

With SMRT (Single Molecule Real-Time) technology, PacBio Sequel enables the real-time detection of DNA synthesis, which is done by incorporation of phospholinked nucleotides, and allows DNA polymerase to complete natural process of elongation. The events of DNA synthesis occurs in the bottom of ZMWs (zero-mode waveguides), allowing the illumination to be identified from background noises such as unincorporated free nucleotides.

PacBio Sequel system provides long-read sequencing, which facilitates assemblies of difficult genomic regions. It also offers benefits such as uniform coverage and high consensus accuracy, and becomes a powerful technology for high quality de novo genome study, full-length transcriptome investigation, haplotype phasing, and long length amplicon research.

 

Roche 454 GS FLX PLUS

It is upgraded to FLX+, the longest read length capacity up to 1kb, generating over 700 Mb to around 1Gb for shotgun genome sequencing. This is very useful for de novo assembly (genome and transcriptome) and long amplicon sequencing studies. There are options of shotgun sequencing for gDNA and RNA, as well as Long Paired-end sequencing designated at 3, 8, and 20 kb.